EpiScreen™ T Cell Epitope Mapping
EpiScreen™ is used to map T cell epitopes within a protein sequence to determine potential for immunogenicity, which is based on the number and potency of T cell epitopes within a sequence. EpiScreen™ T cell epitope mapping typically uses CD8+ T cell depleted PBMCs from a minimum of 50 HLA-typed donors (selected to represent the human population of interest). Typically, 15mer peptides with 12 amino acid overlaps spanning a protein sequence are analyzed in a large number of replicate cultures for in vitro CD4+ T cell stimulation by 3H TdR incorporation. CD4+ T cell stimulation is often detected in two or three adjacent and overlapping peptides since the core 9mer that binds the MHC class II binding groove will be present in more than one peptide sequence. After the accurate identification of peptides that stimulate CD4+ T cells in vitro, In Silico Technologies can be used to design epitope-depleted (deimmunised) variants by determining the precise location of core 9mer sequences and the location of key MHC class II anchor residues.