In silico technologies
EpiScreen™ - T cell epitope mapping
EpiScreen™ can be used to map T cell epitopes within a protein to determine its potential immunogenicity. EpiScreen™ T cell epitope mapping has been used to determine the precise location, number and magnitude of T cell epitopes in many toxins, protein scaffolds and proteins (both human and non-human) prior to deimmunisation.
The assay typically uses CD8+ T cell depleted peripheral mononuclear blood cells (PBMCs) from a minimum of 50 HLA-typed donors, selected to represent the human population of interest, and overlapping short peptides spanning the protein sequence. CD4+ T cell activation is determined by measuring T cell proliferation using 3H-thymidine uptake.
After the accurate identification of peptides that stimulate CD4+ T cells in vitro, iTope™ and TCED™, our in silico tools, can be used to design deimmunised variants.
For further information, please contact us.
1 Baker MP and Jones TD. Identification and removal of immunogenicity from therapeutic proteins. Current Opinion Drug Discovery Development. (2007); 10(2):219-227